Journal: Discovery Medicine

Article Title: Establishment of Transgenic Mouse Leukemia Cell Lines Expressing Human CD4/CCR5/CyclinT1 Infected with HIV-1

doi: 10.24976/discov.med.202335175.12

Figure Lengend Snippet: Fig. 2. Transfection efficiency at different MOIs transfection. L1210 cells were transfected with different lentiviruses MOIs.

Article Snippet: HEK293T cell line and mouse leukemia cell line L1210 were obtained from the ATCC (American Type Culture Collection) library.

Techniques: Transfection

Journal: Discovery Medicine

Article Title: Establishment of Transgenic Mouse Leukemia Cell Lines Expressing Human CD4/CCR5/CyclinT1 Infected with HIV-1

doi: 10.24976/discov.med.202335175.12

Figure Lengend Snippet: Fig. 3. CD4, CCR5, and CyclinT1 levels in transgenic cells qRT-PCR analysis. L1210 cell was transfected with different

Article Snippet: HEK293T cell line and mouse leukemia cell line L1210 were obtained from the ATCC (American Type Culture Collection) library.

Techniques: Transgenic Assay, Quantitative RT-PCR, Transfection

Journal: Discovery Medicine

Article Title: Establishment of Transgenic Mouse Leukemia Cell Lines Expressing Human CD4/CCR5/CyclinT1 Infected with HIV-1

doi: 10.24976/discov.med.202335175.12

Figure Lengend Snippet: Fig. 4. CD4, CCR5, and CyclinT1 protein expression in transgenic L1210 cells. L1210 cells transfected with lentivirus containing CD4/CCR5/CyclinT1 expressed CD4, CCR5, and CyclinT1. ∗p < 0.05 and ∗∗p < 0.01.

Article Snippet: HEK293T cell line and mouse leukemia cell line L1210 were obtained from the ATCC (American Type Culture Collection) library.

Techniques: Expressing, Transgenic Assay, Transfection

Journal: Discovery Medicine

Article Title: Establishment of Transgenic Mouse Leukemia Cell Lines Expressing Human CD4/CCR5/CyclinT1 Infected with HIV-1

doi: 10.24976/discov.med.202335175.12

Figure Lengend Snippet: Fig. 5. HIV-1 RNA levels in transgenic L1210 cells. L1210 cells transfected with the lentivirus vector containing CD4/CCR5/CyclinT1

Article Snippet: HEK293T cell line and mouse leukemia cell line L1210 were obtained from the ATCC (American Type Culture Collection) library.

Techniques: Transgenic Assay, Transfection, Plasmid Preparation

Journal: Discovery Medicine

Article Title: Establishment of Transgenic Mouse Leukemia Cell Lines Expressing Human CD4/CCR5/CyclinT1 Infected with HIV-1

doi: 10.24976/discov.med.202335175.12

Figure Lengend Snippet: Fig. 6. HIV-1 RNA expression level in CEMx174 cells. (A) The HIV-1 RNA level in the supernatant. (B) Cellular HIV-1 RNA expres- sion. H-M represents cells infected with the HIV-1 collected from transgenic L1210, negative control represents cells infected with the

Article Snippet: HEK293T cell line and mouse leukemia cell line L1210 were obtained from the ATCC (American Type Culture Collection) library.

Techniques: RNA Expression, Infection, Transgenic Assay, Negative Control

Journal: PLoS ONE

Article Title: Intrinsic growth heterogeneity of mouse leukemia cells underlies differential susceptibility to a growth-inhibiting anticancer drug

doi: 10.1371/journal.pone.0236534

Figure Lengend Snippet: (A and B) Schematic representation of the device. (A) The top view of the device. The culture medium flows through the trench (white arrows). Cells trapped in the growth channels are observed simultaneously by time-lapse imaging. (B) A cross-section of the device at the plane corresponding to the broken line indicated by the black arrows in (A). The height and the width of a growth channel are both 20 μm, which corresponds to the size of L1210 cells. (C) A micrograph of L1210 cells in the device. (D) Tracing individual L1210 cell lineages. Twenty representative single-cell lineages of L1210 cells cultured in a constant environment are shown. Each horizontal line represents a single-cell lineage, and the points represent the time points at which cell division occurred. The blue cell lineages are fast-cycling cell lineages, which divided 12 times or more during the seven-day culture; the red cell lineages are slow-cycling cell lineages, which divided 11 times or less during the same period. Co-existence of the heterogeneous growth phenotype is further discussed in the main text. (E) Constant division rate. At each time point t , we estimated an instantaneous cell division probability as D ( t ) N ( t ) , where N ( t ) is the number of surviving cells, and D ( t ) is the number of cells that underwent cell division within the time-lapse interval ( t , t + Δ t ]. Δ t is 10 min throughout this study. The vertical axis is the cumulative sum of instantaneous division probability, i.e., ∑ t ′ = 0 t D ( t ′ ) N ( t ′ ) . Blue points represent the experimental data. The black broken-line is the line of linear fit; the slope is (9.033±0.003) × 10 −2 h −1 . The cumulative sum of division probability increases linearly with t , which indicates that the division rate was constant throughout the observation period. (F) Constant death rate. The fraction of surviving cell lineages was plotted over time with the vertical axis on the log scale. Blue points are the experimental data. Black broken-line is the line of linear fit; the slope is −(2.239±0.009) × 10 −3 h −1 . The surviving fraction decreased in an exponential manner, which indicated a constant death rate throughout the observation period.

Article Snippet: L1210 mouse lymphocytic leukemia cells were purchased from ATCC (ATCC CCL-219).

Techniques: Imaging, Cell Culture

Journal: PLoS ONE

Article Title: Intrinsic growth heterogeneity of mouse leukemia cells underlies differential susceptibility to a growth-inhibiting anticancer drug

doi: 10.1371/journal.pone.0236534

Figure Lengend Snippet: (A) Decrease of division rate upon drug exposure. The cumulative sum of instantaneous division probability of L1210 cells in the microfluidic device was plotted over time. In these experiments, MMC exposure started at 96 h (vertical broken line). Blue points represent the result of exposure to 50 nM MMC and green points represent the result of exposure to 200 nM MMC. In both cases, decreases in the division probability started immediately after the drug exposure (around 100 h). We tracked 287 and 211 single-cell lineages (growth channels) in the experiments with 50 nM and 200 nM MMC, respectively, among which 210 and 121 lineages were still surviving at the onset of drug exposure. (B) Increase in death rate on drug exposure. The fraction of surviving cell lineages was plotted over time. In both low and high dosage treatment, death rates started to increase around 150 h. (C and D) Division and death events within single-cell lineages. Panel C represents growth under 50 nM MMC, and Panel D represents growth under 200 nM MMC. The gray-shaded time windows represent the periods of drug exposure. Circles represent cell divisions, and crosses cell death. Red lineages represent fast-cycling cell lineages that underwent seven or more divisions during four days of culture before drug exposure; and blue lineages represent slow-cycling cell lineages that underwent six or fewer divisions during the same period. (E and F) Survival curves for fast-cycling (red) and slow-cycling (blue) cell lineages. Shaded regions around the curves represent standard errors. Panel E represents the result of exposure to 50 nM MMC and Panel D represents the result of exposure to 200 nM MMC.

Article Snippet: L1210 mouse lymphocytic leukemia cells were purchased from ATCC (ATCC CCL-219).

Techniques: